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  • Premium crosslinked allophycocyanin ideal for cell staining, imaging applications and flow cytometry

Fluodyn™ CrossLinked Allophycocyanin Pure Grade

In stock

CatalogSizeFormPrice
APX-PPG-1010M10 mgPrecipitated€208.00
APX-LPG-1010M10 mgLyophilized€291.40
APX-PPG-1050M50 mgPrecipitated€796.80
APX-LPG-1050M50 mgLyophilized€1,115.50
APX-PPG-1100M100 mgPrecipitated€1,049.00
APX-LPG-1100M100 mgLyophilized€1,468.00

OVERVIEW

Fluodyn™ Allophycocyanin (APC) is a water-soluble fluorescent pigment protein extracted from Spirulina thanks to an innovative proccess developped by Biosciencia®.

Allophycocyanin is a part of the photosynthetic machinery of cyanobacteria and its structure optimizes the capture and transfer of light energy. When the individual molecules are purified and isolated, they become highly fluorescent. Due to its high quantum yield and absorbance/fluorescent properties, APC offers a wide range of abilities as immunological assays, protein labelling and cell sorting.

Labeling of macromolecules with APC can provide absorbance coefficients 30-fold higher than with small synthetic fluorophores. Fluodyn™ Allophycocyanin is proposed under precipited or lyophilized form. Precipited form is provided with Quick Desalting System ready to use in few minutes. It can be conjugate with a wide range of molecules using standard reageant.

SPECIFICATIONS

FromSpirulina sp.
Exitation650nm +/- 2nm
Emission660nm +/- 2nm
Quantum Yield0.68
Molecular Weight104 kDa
Extinction Coefficient70
PurityAλmax/A280 > 4.2
Aλmax/A620 > 1.25
Storage4°C/39°F Avoid light and freezing
Shelf life1 year

REFERENCES

  • Glazer, A. N. Phycobilisomes: structures and dynamics. Ann Rev Microbiol 36 :173–198 (1982)
  • Kronick, M. N.The use of phycobiliproteins as fluorescent labels in immunoassay. JImm Meth 92: 1 – 13 (1986)
  • MacColl,R. and D.Guard-Friar.Phycobiliproteins.CRCPress,Inc.,Boca Raton,Florida (1987)
  • MacColl, R., K. Csatorday, D. Berns and E. Traeger.The relationship of the quaternary structure of allophycocyanin to its spectrum. Arch BiochemBiophys  208(1):42 -48 (1981)

For in vitro research use only. Not for diagnostic or therapeutic procedures.

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