Fluodyn™ Allophycocyanin (APC) is a water-soluble ﬂuorescent pigment protein extracted from Spirulina thanks to an innovative proccess developped by Biosciencia®.
Allophycocyanin is a part of the photosynthetic machinery of cyanobacteria and its structure optimizes the capture and transfer of light energy. When the individual molecules are puriﬁed and isolated, they become highly ﬂuorescent. Due to its high quantum yield and absorbance/ﬂuorescent properties, APC oﬀers a wide range of abilities as immunological assays, protein labelling and cell sorting.
Labeling of macromolecules with APC can provide absorbance coeﬃcients 30-fold higher than with small synthetic ﬂuorophores. Fluodyn™ Allophycocyanin is proposed under precipited or lyophilized form. Precipited form is provided with Quick Desalting System ready to use in few minutes. It can be conjugate with a wide range of molecules using standard reageant.
From Spirulina sp. Exitation 650nm +/- 2nm Emission 660nm +/- 2nm Quantum Yield 0.68 Molecular Weight 104 kDa Extinction Coefficient 70 Purity
Aλmax/A280 > 4.2
Aλmax/A620 > 1.25
Storage 4°C/39°F Avoid light and freezing Shelf life 1 year
- Glazer, A. N. Phycobilisomes: structures and dynamics. Ann Rev Microbiol 36 :173–198 (1982)
- Kronick, M. N.The use of phycobiliproteins as fluorescent labels in immunoassay. JImm Meth 92: 1 - 13 (1986)
- MacColl,R. and D.Guard-Friar.Phycobiliproteins.CRCPress,Inc.,Boca Raton,Florida (1987)
- MacColl, R., K. Csatorday, D. Berns and E. Traeger.The relationship of the quaternary structure of allophycocyanin to its spectrum. Arch BiochemBiophys 208(1):42 -48 (1981)
For in vitro research use only. Not for diagnostic or therapeutic procedures.