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Fluodyn™ B-Phycoerythrin Pure Grade

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Fluodyn™ B-Phycoerythrin Pure Grade

Availability: In stock

Brightness
Brightest
Photostability
Med. Low
Max Ex.
546nm
Max Em.
576nm
Laser Line
532-561
Filter
575/26

Catalog

Size

Form

Quantity

Price

BPE-PPG-1010M

10 mg

Precipitated

€119.00

BPE-LPG-1010M

10 mg

Lyophilized

€166.60

BPE-PPG-1050M

50 mg

Precipitated

€454.80

BPE-LPG-1050M

50 mg

Lyophilized

€636.72

BPE-PPG-1100M

100 mg

Precipitated

€598.80

BPE-LPG-1100M

100 mg

Lyophilized

€838.32
OR
Description

Details

OVERVIEW

Fluodyn™ B-Phycoerythrin (B-PE) is a water-soluble fluorescent pigment protein extracted from Porphyridium thanks to an innovative proccess developped by Biosciencia®.

Thanks to its extremely high quantum yield, B-Phycoerythrin is the best choice for flow cytometry.It has the specificity to be low reactive toward non-targetted materials, conferring high selectivity abilities and low background generation.However, B-Phycoerythrin is sensitive to photobleaching and consequently not the best choice for fluorescent microscopy applications.

B-Phycoerythrin is a part of the photosynthetic machinery of microalga and its structure optimizes the capture and transfer of light energy. When the individual molecules are purified and isolated, they become highly fluorescent. Due to its high quantum yield and absorbance/fluorescent properties, B-PE offers a wide range of abilities as immunological assays, protein labelling and cell sorting.

Labeling of macromolecules with B-PE can provide absorbance coefficients 30-fold higher than with small synthetic fluorophores. Fluodyn™B-Phycoerythrin is proposed under precipited or lyophilized form. Precipited form is provided with Quick Desalting System ready to use in few minutes. It can be conjugate with a wide range of molecules using standard reageant.

SPECIFICATIONS

From Porphyridium sp.
Exitation 546nm +/- 2nm
Emission 576nm +/- 2nm
Quantum Yield 0.98
Molecular Weight 240 kDa
Extinction Coefficient 110
Purity

Aλmax/A280 > 5.5

A620/Aλmax < 0.01

A560/A496 < 2.7

Storage 4°C/39°F Avoid light and freezing

DOCUMENTATION

For in vitro research use only. Not for diagnostic or therapeutic procedures.