- Description
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Details
OVERVIEW
Fluodyn™ Allophycocyanin (APC) is a water-soluble fluorescent pigment protein extracted from Spirulina thanks to an innovative proccess developped by Biosciencia®.
Allophycocyanin is a part of the photosynthetic machinery of cyanobacteria and its structure optimizes the capture and transfer of light energy. When the individual molecules are purified and isolated, they become highly fluorescent. Due to its high quantum yield and absorbance/fluorescent properties, APC offers a wide range of abilities as immunological assays, protein labelling and cell sorting.
Labeling of macromolecules with APC can provide absorbance coefficients 30-fold higher than with small synthetic fluorophores. Fluodyn™ Allophycocyanin is proposed under precipited or lyophilized form. Precipited form is provided with Quick Desalting System ready to use in few minutes. It can be conjugate with a wide range of molecules using standard reageant.
SPECIFICATIONS
From Spirulina sp. Exitation 650nm +/- 2nm Emission 660nm +/- 2nm Quantum Yield 0.68 Molecular Weight 104 kDa Extinction Coefficient 70 Purity Aλmax/A280 > 4.2
Aλmax/A620 > 1.25
Storage 4°C/39°F Avoid light and freezing Shelf life 1 year DOCUMENTATION
REFERENCES
- Glazer, A. N. Phycobilisomes: structures and dynamics. Ann Rev Microbiol 36 :173–198 (1982)
- Kronick, M. N. The use of phycobiliproteins as fluorescent labels in immunoassay. J Imm Meth 92:1–13 (1986)
- McCartney, L. J. et al. Near-Infrared Fluorescence Lifetime Assay for Serum Glucose Based on Allophycocyanin-Labeled Concanavalin A. Anal. Biochem. 292:216-221(2001)
For in vitro research use only. Not for diagnostic or therapeutic procedures.
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